Regulatory
Pspac-hy

Part:BBa_I746665:Design

Designed by: Yue Miao   Group: iGEM07_Cambridge   (2007-10-24)

Pspac-hy promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a G->T point mutation of Pspac at the -1 site, changing the promoter to a stronger one - hence the name Pspac-hy, hy meaning hyper. This promoter drives transcription at rates of about ten times that of the wild type (Pspac) promoter.

Source

Comes from the pPL82 vector, which is intended as a B. subtilis integrational vector. It is placed in front of the MCS in the plasmid, as it would drive the expression of the gene cloned into the MCS both in B. subtilis and E. coli.

References

  • Yansura DG, Henner DJ. 1984. Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis. Proc Natl Acad Sci USA. 1984 Jan;81(2):439-43. ([http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6420789&dopt=AbstractPlus])
  • Quisel JD, Burkholder WF, Grossman AD. In vivo effects of sporulation kinases on mutant Spo0A proteins in Bacillus subtilis. J Bacteriol. 2001 Nov;183(22):6573-8. ([http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=11673427])